Tag
cln2

Part:BBa_K105014:Design

Designed by: Robin Sorg   Group: iGEM08_ESBS-Strasbourg   (2008-10-19)

cln2 PEST destabilization domain for rapid protein turnover


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n>

For more information about this issus, see:<br\n>

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

Yeast genomic DNA

References

S. R. Salama, K. B. Hendricks & J. Thorner (1994) Mol Cell Biol 14(12) 7953-7966.